MMSL 2022, 91(4):318-323 | DOI: 10.31482/mmsl.2022.007

THE CORRELATION BETWEEN L-CARNITINE UPTAKE AND SOME HEMATOLOGICAL PARAMETERS IN OXIDATIVE STRESSED RATSOriginal article

Omar B. Aghaa ORCID...1*, Hind T. Hamad ORCID...2
1 College of Pharmacy, University of Mosul, Almajmoa street, Mosul, Ninevah Province, Iraq
2 Al-Dour Technical Institute, Northern Technical University, Salahaddin Province, Iraq

The objective of this research was to determine the effect of L-carnitine antioxidant ability on some hematological parameters such as the extent of red blood corpuscles (RBCs), "Packed cells volume (PCV)", Hemoglobin (Hb), and Mean cellular volume (MCV) in normal and oxidative stress exposed rats respectively. Using an animal model, "Hydrogen peroxide ((H2O2 0.5 %)" was mixed with drinking tap water to induce oxidative stress through the experiment period (30 days). Three groups were used in this study (each containing 7 rats) one of them was the negative control group and the other two were the positive control group (oxidative stress group treated with H2O2) and the treatment group treated with H2O2 + L-carnitine. The oxidative stress group showed a significant decrease (P<0.05) in RBCs count, and no significant differences (P<0.05) were observed in Hb, PCV, and MCV compared with the control group on the other hand the treatment group revealed a significant increase (P<0.05) in RBCs count in cooperation with the oxidative stress group. It can be concluded that L-carnitine has a positive key role toward H2O2 induced oxidative stress. This role positively affects the red bone marrow and erythropoiesis through balancing the negative effect of oxidative stress and can be monitored through RBCs count only.

Keywords: L-carnitine; Oxidative stress; RBCs count; Haemoglobin; H2O2

Received: December 16, 2021; Revised: January 27, 2022; Accepted: January 31, 2022; Prepublished online: March 16, 2022; Published: December 2, 2022  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Aghaa, O.B., & Hamad, H.T. (2022). THE CORRELATION BETWEEN L-CARNITINE UPTAKE AND SOME HEMATOLOGICAL PARAMETERS IN OXIDATIVE STRESSED RATS. MMSL, 91(4), 318-323. doi: 10.31482/mmsl.2022.007
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aruoma OI, Grootveld M, Bahorun T. Free radicals in biology and medicine: from inflammation to biotechnology. Biofactors. 2006;27(1-4):1-3. doi: 10.1002/biof.5520270101. Go to original source... Go to PubMed...
    2. Yoshikawa T, Naito Y. What is oxidative stress?. Japan medical association journal. 2002 Jul;45(7):271-6. doi: 10.1016/S0026-0495(00)80077-3. Go to original source... Go to PubMed...
    3. Kábelová A, Malínská H, Marková I, et al. Ellagic acid affects metabolic and transcriptomic profiles and attenuates features of metabolic syndrome in adult male rats. Nutrients. 2021 Mar;13(3):804. doi: 10.1590/1806-9282.63.01.85. Go to original source... Go to PubMed...
    4. Whaley-Connell A, McCullough PA, Sowers JR. The role of oxidative stress in the metabolic syndrome. Reviews in cardiovascular medicine. 2011 Mar 30;12(1):21-9. DOI: 10.3909/ricm0555. Go to original source... Go to PubMed...
    5. Dayanand CD, Krishnamurthy N, Ashakiran S, et al. Carnitine: a novel health factor-an overview. Int J Pharm Biomed Res. 2011;2(2):79-89.
    6. Servillo L, Giovane A, Cautela D, et al. Where does Nε-trimethyllysine for the carnitine biosynthesis in mammals come from?. PloS one. 2014 Jan 13;9(1):e84589. doi: 10.1371/journal.pone.0084589. Go to original source... Go to PubMed...
    7. Scheen AJ. Cardiovascular risk-benefit profile of sibutramine. American Journal of Cardiovascular Drugs. 2010 Oct;10(5):321-34. doi: 10.2174/138920011796504536. Go to original source... Go to PubMed...
    8. Kim A, Nemeth E. New insights into iron regulation and erythropoiesis. Current opinion in hematology. 2015 May;22(3):199. doi: 10.1097/MOH.0000000000000132 Go to original source... Go to PubMed...
    9. Level DF. Subject to Genetic Variation BCL11A An Erythroid Enhancer of. Nat. Rev. Microbiol. 2010;8:15-25. doi: 10.1126/science.1242088. Go to original source... Go to PubMed...
    10. Koury MJ, Ponka P. New insights into erythropoiesis: the roles of folate, vitamin B12, and iron. Annu. Rev. Nutr.. 2004 Jul 14;24:105-31. doi:10.1146/annurev.nutr.24.012003.132306. Go to original source... Go to PubMed...
    11. Hero M, Wickman S, Hanhijärvi R, et al. Pubertal upregulation of erythropoiesis in boys is determined primarily by androgen. The Journal of pediatrics. 2005 Feb 1;146(2):245-52. doi: 10.1016/j.jpeds.2004.09.002. Go to original source... Go to PubMed...
    12. Birkmann J, Oez S, Smetak M, et al. Effects of recombinant human thrombopoietin alone and in combination with erythropoietin and early-acting cytokines on human mobilized purified CD34+ progenitor cells cultured in serum-depleted medium. Stem Cells. 1997 Jan;15(1):18-32. doi: 10.1002/stem.150018. Go to original source... Go to PubMed...
    13. Menon MP, Karur V, Bogacheva O, et al. Signals for stress erythropoiesis are integrated via an erythropoietin receptor-phosphotyrosine-343-Stat5 axis. The Journal of clinical investigation. 2006 Mar 1;116(3):683-94. doi: 10.1172/JCI25227. Go to original source...
    14. Koury MJ, Koury ST, Kopsombut P, et al. In vitro maturation of nascent reticulocytes to erythrocytes. blood. 2005 Mar 1;105(5):2168-74. doi: 10.1182/blood-2004-02-0616. Go to original source... Go to PubMed...
    15. Kryston TB, Georgiev AB, Pissis P, et al. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. Mutat. Res., Fundam. Mol. Mech. Mutagen. 2011;711(1-2):193-201. doi: 10.1016/j.mrfmmm.2010.12.016. Go to original source... Go to PubMed...
    16. Pieni±¿ek D, Bukowska B, Duda W. Comparison of the effect of Roundup Ultra 360 SL pesticide and its active compound glyphosate on human erythrocytes. Pesticide biochemistry and physiology. 2004 Jun 1;79(2):58-63. doi:10.1016/j.pestbp.2004.03.003. Go to original source...
    17. Baskurt OK, Temiz A, Meiselman HJ. Effect of superoxide anions on red blood cell rheologic properties. Free Radical Biology and Medicine. 1998 Jan 1;24(1):102-10. doi: 10.1016/S0891-5849(97)00169-X. Go to original source... Go to PubMed...
    18. Chien S. Red cell deformability and its relevance to blood flow. Annual review of physiology. 1987 Mar;49(1):177-92. doi: 10.1146/annurev.physiol.49.1.177. Go to original source... Go to PubMed...
    19. Chasis JA, Shohet SB. Red cell biochemical anatomy and membrane properties. Annual review of physiology. 1987 Mar;49(1):237-48. doi: 10.1146/annurev.ph.49.030187.001321. Go to original source... Go to PubMed...
    20. Friederichs E, Farley RA, Meiselman HJ. Influence of calcium permeabilization and membrane-attached hemoglobin on erythrocyte deformability. American journal of hematology. 1992 Nov;41(3):170-7. doi: 10.1002/ajh.2830410306. Go to original source... Go to PubMed...
    21. Nollet LM, Toldrá F, editors. Handbook of analysis of active compounds in functional foods. CRC Press; 2012 Jan 18. Go to original source...
    22. Kitamura Y, Satoh K, Satoh T, et al. Effect of L-carnitine on erythroid colony formation in mouse bone marrow cells. Nephrology Dialysis Transplantation. 2005 May 1;20(5):981-4. doi: 10.1093/ndt/gfh758. Go to original source... Go to PubMed...
    23. Arduini A, Rossi M, Mancinelli G, et al. Effect of L-carnitine and acetyl-L-carnitibe on the human erythrocyte membrane stability and deformability. Life sciences. 1990 Jan 1;47(26):2395-400. doi: 10.1016/0024-3205(90)90483-8. Go to original source... Go to PubMed...
    24. Van Gestel S, Forsgren T, Claes S, et al. Epistatic effect of genes from the dopamine and serotonin systems on the temperament traits of novelty seeking and harm avoidance. Molecular Psychiatry. 2002 Jun;7(5):448-50. doi: 10.1038/sj.mp.4000805. Go to original source... Go to PubMed...
    25. Al-Majed AA, Sayed-Ahmed MM, Al-Yahya AA, et al. Propionyl-L-carnitine prevents the progression of cisplatin-induced cardiomyopathy in a carnitine-depleted rat model. Pharmacological research. 2006 Mar 1;53(3):278-86. doi: 10.1016/j.phrs.2005.12.005. Go to original source... Go to PubMed...
    26. Rani PJ, Panneerselvam C. Carnitine as a free radical scavenger in aging. Experimental gerontology. 2001 Nov 1;36(10):1713-26. doi: 10.1016/S0531-5565(01)00116-4. Go to original source... Go to PubMed...
    27. Bertelli A, Conte A, Ronca G. L-propionyl carnitine protects erythrocytes and low density lipoproteins against peroxidation. Drugs under experimental and clinical research. 1994 Jan 1;20(5):191-7. Go to PubMed...
    28. Calvani M. Benatti P, Mancinelli A, et al. Carnitine replacement in end-stage renal disease and hemodialysis. Ann NY Acad Sci. 2004;1033:52-66. doi: 10.1196/annals.1320.005. Go to original source... Go to PubMed...

    Return to the content